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RAMOS E/M Series
SPECIFICATION
| RAMOS E200 | RAMOS M350 | RAMOS M520 | RAMOS M750 | ||||
| Image acquisition mode: | 3D (XYZ) confocal laser and Raman images | ||||||
Scanning type: | XY galvano-mirror scanners XY motorized stage (option) Z piezo scanner | ||||||
| Scanning speed: | 1000 х 1000 pixels per 3 sec (3 μs/pixel) | ||||||
| Spatial resolution: | XY: 440 nm, Z: 620 nm (532 nm laser, 100 x , NA = 0.95) | ||||||
| Spectral range: | 50 – 8500 cm-1 (532 nm laser) | 50 – 9700 cm-1 (532 nm laser) | |||||
| Excitation source: | Build-in 473 nm or 532 nm laser, connection of additional external lasers 455 nm, 633 nm, 785 nm is possible | ||||||
| Laser beam attenuator: | Automated unit with VND filter, continuously changeable from 0.1% to 100% | ||||||
| Rayleigh rejection filters: | A pair of Edge filters with cut-off starting from 50 cm-1 (for 532 nm laser) | ||||||
| Spectrometer configuration: | 2-channel imaging spectrometer directly coupled to a microscope | External imaging spectrograph | |||||
| Focal length: | 200 mm | 350 mm | 520 mm | 750 mm | |||
Spectral resolution | 1 cm-1/pixel (grating 2400 gr/mm) 2 cm-1/pixel, FWHM (grating 2400 gr/mm) | 1.60 cm–1/pixel (grating 1800 groove/mm) | 0.76 cm -1/pixel (grating 1800 groove/mm) 0.25 cm -1/pixel (Echelle grating) | 0.44 cm -1/pixel (grating 1800 groove/mm) | |||
Number of gratings: | 2 gratings, 4 optionally | 4 mounted on motorized turret, more gratings with manual changing | |||||
| Detection system: | CCD sensor 2048х122 pixels, with Peltier cooling | CCD sensor 2048х122 with two-stage cooling, quantum efficiency up to 95% | |||||
| Options: | Heating & cooling stages, fiber optic probe, | ||||||
FLIM option is available.
Combination with AFM is possible.
3D scanning laser Raman microscopes provide rapid, high sensitivity analysis and unprecedented convenience in use
RAMOS E/M Series Raman spectrometers are designed on the basis of research grade optical microscopes allowing the realization of the following light microscopy methods:
- Raman measurements
- Transmitted light
- Reflected light (bright field and dark field illumination)
- Confocal microscopy
- Fluorescence measurements
- Polarization contrast and phase contrast imaging
- Differential interference contrast
The innovative approach to system design of Raman spectrometers ensures extremely high temperature and temporal stability of spectral measurements.
All components of RAMOS E200 system are fully integrated within an optical microscope providing compactness and mobility of the system.
In RAMOS M350, M520, M750 systems external imaging spectrographs are connected via optical fibers.
Raman measurements with the RAMOS E/M Series systems can be started in several minutes by turning a system key.
1. High resolution colour video camera for a sample observation
2. Optical microscope
3. Raman optical module
4. Automated 3–position turret with mirrors for input / output radiation
5. Confocal laser microscope module (“Reflection” module)
6. Two-channel imaging spectrometer
7. XY galvano mirror scanner
8. Z piezo scаnner
9. Automated microscope stage
Confocal Raman microscope with scanning speed of 1000 x 1000 pixels within total acquisition time of 3 sec
Fast raster and start-stop scanner operational modes
Spatial resolution of confocal images (100x, NA = 0.95)
Laser wavelength, nm | XY – plane resolution, nm | Z (axial) resolution, nm |
| 473 | 390 | 550 |
| 532 | 440 | 620 |
| 633 | 520 | 730 |
| 785 | 650 | 910 |
Simultaneous 2D / 3D laser confocal and Raman imaging with full spectra saving in each point
Confocal imaging of deep sample layers
Ultra-fast scanning with Galvano-mirrors
High-performance optics ensures super system sensitivity and high signal-to-noise ratio (S/N ratio)
Wide spectral range, ultimate accuracy of Raman shift measurements, high spectral resolution
Fast system configuration changing (lasers and filters switching) without additional system alignment
Extremely sensitive detectors for Raman signal registration
- Spectral CCD camera provides outstanding performance due to the increased number of pixels (the number of pixels is 2048) and the smaller pixel pitches (12 x 12 μm)
- Photodetector quantum efficiency is up to 95%. Low dark signal level due to two-stage thermoelectric sensor cooling
- Detectors operating internal memory with the capacity of up to 128 MB
- High-speed Ethernet interface for data transfer
The system may be completed with the following options:
- Automated XY stage for an ultra wide field of scanning
- High temperature and vacuum cells
- Cryostat
- Remote fiber probe
High-precision spectrometer calibration
RAMOS E/M Series spectrometers can be optionally equipped with a neon lamp for high-accuracy spectral calibration
RAMOS M350
Compact confocal Raman microscope RAMOS M350 is intended for various spectral measurements with submicrometer scale image resolution. The device features high throughput and a high spectral resolution.
RAMOS M520
Confocal Raman microscope RAMOS M520 with high-end class capabilities is applicable for spectral measurements with sub micrometer scale image resolution. The device possesses all features of M350 but ensures the enhanced spectral resolution
RAMOS M750
Confocal Raman microscope RAMOS M750 is perfect for spectral measurements with the extremely high spectral resolution. Up to two detectors can be used simultaneously in the above three system models.
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